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Image Search Results
Journal: medRxiv
Article Title: Pathogenic CD8 T cells defined by longitudinal liver sampling in chronic hepatitis B patients starting antiviral therapy
doi: 10.1101/2021.12.16.21267870
Figure Lengend Snippet: (A) IHMC from 6 healthy donors were treated with IL-2, IL-12, or IL-2 + IL-12 for 24 h before quantification of IFNγ and Fas ligand expression of CXCR6(+) CD8 T cells by flow cytometry. Statistical significance was assessed by paired t test. (B) A multi-color flow cytometry panel was used to analyze IHMC from 6 healthy donors, without and with 24 h treatment with IL-2 + IL-12. Single, live, CD3(+) lymphocytes were selected before clustering. The flare CD8 T population, corresponding to baseline FNA samples from CHB patients, was only found in the treated healthy donor IHMC. Heatmaps show median expression. (C) Source of IL-2 and IL-12 in CHB patients’ livers at baseline. A targeted scRNAseq assay was used to enrich for cytokine genes. Both IL-2 and IL-12A/IL-12B could be detected.
Article Snippet: Indicated cytokines were used at the following concentrations:
Techniques: Expressing, Flow Cytometry